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Nanocrystalline cellulose/β-casein conjugated nanoparticles prepared by click chemistry
- Karaaslan, Muzaffer A., Gao, Guangzheng, Kadla, John F.
- Cellulose 2013 v.20 no.6 pp. 2655-2665
- acetylene, acid hydrolysis, beta-casein, cellulose, chemistry, drug delivery systems, immunoassays, nanomedicine, nanoparticles
- Functionalized nanoparticles are promising building blocks for well-defined nanomaterials with unique properties. Site-specific or regio-selective functionalization of those nanoparticles and organization into high-order assemblies is a major challenge in materials research. Here, we demonstrate site-specific immobilization of a model protein at one tip of nanocrystalline cellulose (NCC), single-crystalline rod-like shaped nanoparticles that are isolated by acid hydrolysis of bulk cellulose. Click reaction between reducing end functionalized NCC bearing azide groups and β-casein micelles bearing acetylene groups results in mushroom-like conjugated nanoparticles in different arrangements. The strategy developed here to design hybrid polysaccharide–protein nanoparticles could be useful for building novel functional self-assembled nanobiomaterials and have potential in nanomedicine, immunoassay and drug delivery applications.