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Full-length sequence analysis of hepatitis E virus isolates: showing potential determinants of virus genotype and identity

Author:
Yang, Dong, Jiang, Mei, Jin, Min, Qiu, Zhigang, Cui, Weihong, Shen, Zhiqiang, Li, Bo, Gong, Lianfeng, Chen, Zhaoli, Wang, Xinwei, Li, Jun-Wen
Source:
Virus genes 2013 v.47 no.3 pp. 414-421
ISSN:
0920-8569
Subject:
Hepatitis E virus, amino acid motifs, amino acids, genotype, nucleotide sequences, phylogeny, sequence analysis, viruses, China
Abstract:
The complete genome sequence of a genotype 4 strain of hepatitis E virus (CH-YT-HEV02) from a patient (in Yantai, China) has been determined. Phylogenetic analysis showed that CH-YT-HEV02 belongs to genotype 4, subtype 4a. However, the phylogenetic analysis indicated that it was most closely related to JKO-CHiSai98C (AB197673) strain, sharing only 91.6 % sequence identity with it. Judging from the phylogenetic tree based on the full-length nucleotide sequences of all 70 genotype 4 HEV isolates retrieved from GenBank up to May, 2013, the CH-YT-HEV02 isolates could serve as a Yantai-indigenous strain. A broader comparison with other genotype isolates revealed that there are a few conserved amino acids in the HVR region of different HEV genotypes, and two amino acid motifs in ORF2 and ORF3 might serve as signatures of genotype diversity of HEV.
Agid:
614212