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Effect of glutathione on sperm quality after short-term cryopreservation in seurukan fish Osteochilus vittatus (Cyprinidae)
- Muthmainnah, C.R., Eriani, K., Hasri, I., Irham, M., Batubara, A.S., Muchlisin, Z.A.
- Theriogenology 2018 v.122 pp. 30-34
- Osteochilus vittatus, analysis of variance, cryopreservation, eggs, fish, glutathione, hatching, sperm motility, sperm quality
- The objective of this present study is to determine the optimum concentration for glutathione in the cryopreservation of seurukan fish (Osteochilus vittatus) spermatozoa. A completely randomized design (CRD) was used with 6 treatments and 3 replications. The Seurukan fish sperm was diluted in extender with a ratio of 1:20 (sperm: extender), then glutathione was added at a concentration of 0, 10, 20, 30, 40 and 50 mg L−1. Next, the sperm thawed at 39–40 °C for 3 min and mixed with 100 eggs which were randomly selected. The success of the fertilized egg was observed 6 h after fertilization, while the hatching rate was examined 60 h after fertilization. The ANOVA test showed that the addition of glutathione significant affected the sperms motility, fertility and hatching rate of seurukan fish Osteochilus vittatus eggs (P < 0.05). By segregation the fresh sperm, a higher sperm motility rate was recorded with an addition of 30 mgL−1 of glutathione (63.00 ± 5.89), however, this value was not significantly different from using concentration of 10 and 20 mg L−1. A higher fertilization rate was produced using glutathione concentration of 50 mg L−1 (51.33 ± 17.01); however, it was also not significantly different from the concentration of 20, 30 and 40 mgL−1. In addition, a higher hatching rate was also recorded using glutathione concentration of 50 mg L−1 (40.33 ± 12.89), this value was not significantly different from using 40 mgL−1 glutathione. Hence, a conclusion was drawn that the optimum concentration of glutathione is 40 mg L−1 of diluent.