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Oligoarginine mediated collagen/chitosan gel composite for cutaneous wound healing

Li, Manman, Han, Mei, Sun, Yusheng, Hua, Yingying, Chen, Guifang, Zhang, Liefeng
International journal of biological macromolecules 2019 v.122 pp. 1120-1127
Fourier transform infrared spectroscopy, Staphylococcus aureus, angiogenesis, antibacterial properties, chitosan, collagen, cytotoxicity, differential scanning calorimetry, gels, granulation tissue, histopathology, mice, physicochemical properties, scanning electron microscopy, therapeutics, tissue repair
In this study, the collagen/chitosan gel composite supplemented with a cell-penetrating peptide (CPP) (Oligoarginine, R8) was prepared. Then, the physicochemical properties of the new collagen/chitosan/CPPs gel obtained were analyzed and the related characteristics were evaluated by scanning electron microscopy (SEM), fourier transform infrared (FTIR), differential scanning calorimetry (DSC), differential thermal analyzer (DTA). Furthermore, we found that collagen/chitosan/CPPs gel composite was capable of inhibiting Staphylococcus aureus growth and had good ability to heal wounds. The mice test results showed that collagen/chitosan/CPPs gel had the highest healing rate, fastest healing speed in all the treatments. After 14 days, the group treated by collagen/chitosan/CPPs gel showed nearly complete wound surface healing rate of 98 ± 4.71%. In addition, histopathological examination suggested that collagen/chitosan/CPPs could promote cutaneous wound healing through enhancing granulation tissue formation, increasing collagen deposition and promoting angiogenesis in the wound tissue. Meanwhile, no significant cytotoxicity of the gel was observed. In conclusion, the collagen/chitosan/CPPs gel composite which has antibacterial activity renders a high therapeutic efficiency to heal wounds.