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Exploring the molecular basis of resistance/ susceptibility to mixed natural infection of Haemonchus contortus in tropical Indian goat breed

Author:
Shrivastava, Kush, Kumar, Pushpendra, Khan, Mohd Faheem, Sahoo, Nihar Ranjan, Prakash, Om, Kumar, Amit, Panigrahi, Manjit, Chauhan, Anuj, Bhushan, Bharat, Prasad, Arvind, Nasir, Abdul, Patel, Bhimnere Hanumanthagouda Manjunatha
Source:
Veterinary parasitology 2018 v.262 pp. 6-10
ISSN:
0304-4017
Subject:
Haemonchus contortus, eggs, enzyme-linked immunosorbent assay, fecal egg count, genes, goats, hematocrit, interleukin-10, loci, messenger RNA, microsatellite repeats, parasites, quantitative trait loci, India
Abstract:
The present investigation was carried out with the objective to identify putative candidate genes / Quantitative trait loci for resistance / susceptibility towards Haemonchus infestation in tropical goat breed (Rohilkhandi goat) of India. The mean faecal egg count (FEC) and packed cell volume (PCV) of the population were 142.78 ± 22.54 epg (eggs per gram) and 31.73% ± 0.49, respectively. Grouping of animals as per dot ELISA test showed 41.33% (n = 124) positive and 58.66% (n = 176) negative for Haemonchus infestation. The microsatellite loci DYA and ODRB1.2 were significantly associated (P ≤ 0.05) to parasite resistance. The locus DYA showed significant association with log FEC and dot ELISA and the locus ODRB1.2 showed significant association with log FEC, PCV and dot ELISA at P ≤ 0.05. Real time expression profiling revealed that the susceptible group (high FEC group) had 11.1-fold more expression of IFNγ mRNA (Th1 cytokine) and 0.11-fold lower expression of IL-10 mRNA (Th2 cytokine), which was found to be statistically significant (P ≤ 0.05).
Agid:
6144816