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Exploring the molecular basis of resistance/ susceptibility to mixed natural infection of Haemonchus contortus in tropical Indian goat breed

Shrivastava, Kush, Kumar, Pushpendra, Khan, Mohd Faheem, Sahoo, Nihar Ranjan, Prakash, Om, Kumar, Amit, Panigrahi, Manjit, Chauhan, Anuj, Bhushan, Bharat, Prasad, Arvind, Nasir, Abdul, Patel, Bhimnere Hanumanthagouda Manjunatha
Veterinary parasitology 2018 v.262 pp. 6-10
Haemonchus contortus, eggs, enzyme-linked immunosorbent assay, fecal egg count, genes, goats, hematocrit, interleukin-10, loci, messenger RNA, microsatellite repeats, parasites, quantitative trait loci, India
The present investigation was carried out with the objective to identify putative candidate genes / Quantitative trait loci for resistance / susceptibility towards Haemonchus infestation in tropical goat breed (Rohilkhandi goat) of India. The mean faecal egg count (FEC) and packed cell volume (PCV) of the population were 142.78 ± 22.54 epg (eggs per gram) and 31.73% ± 0.49, respectively. Grouping of animals as per dot ELISA test showed 41.33% (n = 124) positive and 58.66% (n = 176) negative for Haemonchus infestation. The microsatellite loci DYA and ODRB1.2 were significantly associated (P ≤ 0.05) to parasite resistance. The locus DYA showed significant association with log FEC and dot ELISA and the locus ODRB1.2 showed significant association with log FEC, PCV and dot ELISA at P ≤ 0.05. Real time expression profiling revealed that the susceptible group (high FEC group) had 11.1-fold more expression of IFNγ mRNA (Th1 cytokine) and 0.11-fold lower expression of IL-10 mRNA (Th2 cytokine), which was found to be statistically significant (P ≤ 0.05).