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Bifunctional Super-resolution Imaging Probe with Acidity-Independent Lysosome-Retention Mechanism

Author:
Xue, Zhongwei, Wang, Siyu, Li, Jian, Chen, Xin, Han, Jiahuai, Han, Shoufa
Source:
Analytical chemistry 2018 v.90 no.19 pp. 11393-11400
ISSN:
1520-6882
Subject:
fluorescence, image analysis, lysosomes, mitophagy, pH, photostability, sialic acid
Abstract:
Spatiotemporal imaging is of enormous use to explore organelle biology, necessitating organelle-tracing techniques reliable in varied cell stress. We herein reported lysosomal imaging using rhodamine-X-integrated sialic acid (ᴿᴼXSA), which is stably maintained in lysosomes irrespective of lysosomal pH changes. Exhibiting bright fluorescence and superior photostability, ᴿᴼXSA enables 120 h continual tracking of fusion/fission of lysosomes and mitochondrion–lysosome interaction in mitophagy. Relative to conventional acidotropic probes prone to dissipation from stressed lysosomes, ᴿᴼXSA offers a new route for long-term tracking of stressed lysosomes relevant to diverse pathological conditions.
Agid:
6145741