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Molecular characterization of a novel vernalization allele Vrn-B1d and its effect on heading time in Chinese wheat (Triticum aestivum L.) landrace Hongchunmai
- Zhang, Bo, Wang, Xianguo, Wang, Xiaolong, Ma, Li, Wang, Zhonghua, Zhang, Xiaoke
- Molecular breeding 2018 v.38 no.10 pp. 127
- Triticum aestivum, alleles, allelic variation, breeding, cultivars, environmental factors, genetic markers, germplasm, greenhouse experimentation, growth habit, introns, landraces, loci, polymerase chain reaction, single nucleotide polymorphism, spring wheat, temperature, vernalization
- Flowering time of wheat cultivars contributes greatly to the adaptability to environmental conditions and it is largely controlled by vernalization genes. In this study, 262 Chinese mini-core wheat cultivars were used to identify the allelic variation at VRN-B1 locus. A novel dominant allele Vrn-B1d was found in Chinese spring wheat landrace cultivar Hongchunmai. This allele contained several genetic divergence within the first intron comparing to the recessive allele vrn-B1, including one large 6850-bp deletion (670–7519 bp), one small 187-bp deletion (7851–8037 bp), one unique SNP (T to C, 7845 bp), and one 4-bp mutation (TTTT to ACAA, 7847–7850 bp). Meanwhile, it was also different from the three known dominant alleles at VRN-B1 locus. Two pairs of primers were designed to identify the novel allele Vrn-B1d and other four known alleles of VRN-B1. A multiplex PCR was established to discriminate all five alleles simultaneously. The greenhouse experiment with high temperature (non-vernalizing condition) and long light showed that F₂ plants containing Vrn-B1d allele headed significantly earlier than those with recessive vrn-B1 allele, suggesting that Vrn-B1d is a dominant allele conferring the spring growth habit. This study provides a useful germplasm and molecular markers for wheat breeding.