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Isoprothiolane or NDGA application affects phytohormone-related gene expressions in 'Shine Muscat' grape berry maturation

Thunyamada, S., Saito, T., Lin, H., Wang, S., Okawa, K., Ohara, H., Kondo, S.
Acta horticulturae 2018 no.1206 pp. 257-262
abscisic acid, auxins, biosynthesis, blast disease, ethylene, flowering, gene expression, genes, grapes, high-throughput nucleotide sequencing, isoprothiolane, messenger RNA, nordihydroguaiaretic acid, plant growth, signal transduction, transactivators
Isoprothiolane (IPT), which is an agent used for rice blast control and plant growth regulation, and an ABA biosynthesis inhibitor, nordihydroguaiaretic acid (NDGA), were applied to 'Shine Muscat' grape berries at 42 days after full bloom (DAFB) (1 week before véraison). Grapes were harvested at 70 DAFB. The RNAs were extracted from the grape skin and analyzed by next-generation sequencing to assemble RNA-seq data. The IPT and NDGA applications both induced auxin-regulated protein 6B-like gene after véraison, and the NDGA application stimulated gibberellin-regulated protein 4 gene. The IPT and NDGA applications both decreased the ethylene-responsive transcription factor 2 gene expression levels. In addition, the IPT and NDGA applications each significantly suppressed the abscisic-aldehyde oxidase-like %2C transcript variant X1 gene expression levels. These results suggest that IPT and NDGA applications may enhance an auxin signaling pathway and gibberellin-related gene activity but suppress ethylene transcriptional activator gene and ABA biosynthesis-related gene expression in grapes.