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Sphingobacterium praediipecoris sp. nov. isolated from effluent of a dairy manure treatment plant

Van Le, Ve, Padakandla, Shalem Raj, Kim, Hyangmi, Chae, Jong-Chan
Archives of microbiology 2018 v.200 no.10 pp. 1481-1486
DNA, Sphingobacterium, bacteria, chemotaxonomy, dairy manure, fatty acids, isoprenoids, new species, nucleotide sequences, pH, phenotype, phosphatidylethanolamines, phylogeny, quinones, ribosomal RNA, sequence homology, sodium chloride
A novel Gram-reaction-negative, rod-shaped, non-motile bacterium, designated as strain G2-10ᵀ was isolated from effluent of a dairy manure treatment plant. Growth occurred at 20–40 °C (optimum at 25–30 °C), pH 7.0–8.0 (optimum at pH 7.0). The range of NaCl concentration for growth was between 0% and 3% (w/v) (optimum 0–1%, w/v). Comparison of 16S rRNA gene sequence indicated that strain G2-10ᵀ was moderately related to the type strains of Sphingobacterium nematocida M-SX103ᵀ and Sphingobacterium suaedae T47ᵀ with a pair-wise sequence similarity of 94.3% and 94.0%, respectively. The major fatty acid constituents of strain G2-10ᵀ were identified as iso-C₁₅:₀ (37.6%), summed feature 3 (consisting of C₁₆:₁ω7c and/or C₁₆:₁ω6c, 29.6%) and iso-C₁₇:₀ 3-OH (15.2%). Phosphatidylethanolamine was the major polar lipids of strain G2-10ᵀ. Sphingophospholipids were present. The isoprenoid quinone was composed of only MK-7. The DNA G + C content of strain G2-10ᵀ was found to be 42.5 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain G2-10ᵀ represents a novel species within the genus Sphingobacterium, for which the name Sphingobacterium praediipecoris is proposed. The type strain is G2-10ᵀ (= KCTC 52880ᵀ = NBRC 112848ᵀ).