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PCR identification and phylogenetic analysis of the medically important dust mite Suidasia medanensis (Acari: Suidasiidae) in Malaysia

Author:
Ernieenor, F. C. L., Ernna, G., Jafson, A. S., Mariana, A.
Source:
Experimental & applied acarology 2018 v.76 no.1 pp. 99-107
ISSN:
0168-8162
Subject:
DNA, Suidasia, acarology, cytochrome-c oxidase, dust, dust mites, genes, light microscopes, mitochondria, nucleotide sequences, phylogeny, polymerase chain reaction, storage mites, taxonomy, Korean Peninsula, Malaysia, United Kingdom
Abstract:
The occurrence of Suidasia medanensis (= S. pontifica) mites in Malaysian house dust was first reported in 1984. The taxonomy of this storage mite is, however, quite confusing. Therefore, we need an accurate identification to resolve morphological problems due to its minute size and some overlapping characters between species. The purpose of this study was to demonstrate the application of partial mitochondrial cytochrome c oxidase subunit I (COI) sequences for the identification of S. medanensis by PCR. Identity of the mite was first determined by observing morphological characters under a light microscope. Genomic DNA of S. medanensis mites was successfully extracted prior to PCR and DNA sequencing using COI universal primers. The length of the COI sequences obtained was 378 bp. BLAST analysis of amplicon sequences showed that local S. medanensis COI region had 99% maximum identity with S. medanensis nucleotide sequence (AY525568) available in the GenBank. As the phylogenetic tree generated indicated, COI sequences from this study were clustered with S. medanensis from Korea and the UK in one major clade, supported with high bootstrap value (> 85%). Results of the phylogenetic analysis of this COI gene were congruent with the morphological identification and provided strong support for a single clade of local S. medanensis.
Agid:
6151314