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Supplementation of Protein Hydrolysates to a Low‐fishmeal Diet Improves Growth and Health Status of Juvenile Olive Flounder, Paralichthys olivaceus

Khosravi, Sanaz, Bui, Hien Thi Dieu, Herault, Mikaël, Fournier, Vincent, Kim, Kyoung‐Duck, Lee, Bong‐Joo, Kim, Kang‐Woong, Lee, Kyeong‐Jun
Journal of the World Aquaculture Society 2018 v.49 no.5 pp. 897-911
Edwardsiella tarda, Paralichthys olivaceus, bacterial infections, dietary supplements, digestibility, disease resistance, enterocytes, experimental diets, feed conversion, fish meal, flounder, goblet cells, growth performance, health status, histology, hydrolysates, immunoglobulins, ingredients, innate immunity, juveniles, krill, lysozyme, protein hydrolysates, respiratory burst, satiety, shrimp, soy protein concentrate
An 11‐wk feeding trial was conducted to evaluate three different protein hydrolysates as feed ingredients in high‐plant‐protein diets for juvenile olive flounder. Five experimental diets were fed to juvenile olive flounder to examine the effect of three different protein hydrolysates on growth performance, innate immunity, and disease resistance against bacterial infection. A basal fishmeal (FM)‐based diet was regarded as a high‐FM diet (HFM) and a diet containing soy protein concentrate (SPC) as a substitute for 50% FM protein was considered as a low‐FM diet (LFM). Three other diets contained three different sources of protein hydrolysates, including shrimp, tilapia, and krill hydrolysates (designated as SH, TH, and KH), replacing 12% of FM protein. All diets were formulated to be isonitrogenous and isocaloric. Triplicate groups of fish (15.1 ± 0.1 g) were handfed one of the diets to apparent satiation twice daily for 11 wk and subsequently challenged against Edwardsiella tarda. Growth performance and feed utilization of fish fed hydrolysate‐supplemented diets were significantly improved compared to those of fish fed the LFM diet. Dietary inclusion of the protein hydrolysates significantly enhanced apparent digestibility of dry matter and protein of the diets. In the proximal intestine, histological alterations were observed in the fish fed the LFM diet. The fish fed the hydrolysate diets showed significantly longer mucosal fold and enterocytes and greater number of goblet cells compared to fish fed the LFM diet. Respiratory burst activity was significantly higher in fish fed the TH and KH diets than fish fed the LFM diet. Significantly higher immunoglobulin levels were found in fish fed SH and KH diets compared to those of fish fed the LFM diet. Dietary inclusion of the protein hydrolysates in SPC‐based diets exhibited the highest lysozyme activity. Significantly higher superoxide activity was observed in groups of fish fed the KH diet. Fish offered the protein hydrolysates were more resistant to bacterial infection caused by E. tarda. The results of this study suggest that the tested protein hydrolysates can be used as potential dietary supplements to improve growth performance and health status of juvenile olive flounder when they were fed a LFM diet.