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Separation of six compounds including two n‐butyrophenone isomers and two stibene isomers from Rheum tanguticum Maxim by recycling high speed counter‐current chromatography and preparative high‐performance liquid chromatography

Author:
Chen, Tao, Yang, Xue, Wang, Nana, Li, Hongmei, Zhao, Jingyang, Li, Yulin
Source:
Journal of separation science 2018 v.41 no.19 pp. 3660-3668
ISSN:
1615-9306
Subject:
Rheum tanguticum, bioactive compounds, countercurrent chromatography, emodin, high performance liquid chromatography, isomers, recycling
Abstract:
Six compounds including two n‐butyrophenone isomers and two stibene isomers were obtained from Rheum tanguticum Maxim. Two n‐butyrophenone isomers with a separation factor of 1.14 were successfully separated by recycling high‐speed counter‐current chromatography after ten cycles. Two stibene isomers were successfully separated by preparative high‐performance liquid chromatography. High‐performance liquid chromatography analysis showed that the purities of the compounds were all over 98%. These compounds were identified as lindleyin, isolindleyin, resveratrol‐4′‐O‐(2″‐O‐galloyl)‐glucopyranoside, resveratrol‐4′‐O‐(6′′‐O‐galloyl)‐glucopyranoside, emodin 1‐O‐β‐d‐glucoside, and 3,5‐dihydroxy‐4′‐methoxystilbene‐3‐O‐β‐d‐glucopyranoside. The results indicated that recycling high‐speed counter‐current chromatography and preparative high‐performance liquid chromatography could be effective combination for the preparation of bioactive compounds from Rheum tanguticum Maxim.
Agid:
6153273