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Immunohistochemical Identification of Generalized AA-Amyloidosis in a Mountain Gazelle (Gazella gazella)

Linke, R. P., Hol, P. R., Geisel, O.
Gazella, amyloid, amyloidosis, animal pathology, antiserum, cattle, cross reaction, disease diagnosis, dogs, enzymes, gazelles, hamsters, humans, immunohistochemistry, mice, polyclonal antibodies
Generalized amyloidosis was diagnosed post-mortem in a mountain gazelle (Gazella gazella). To test whether the amyloid deposits consisted of amyloid-A fibril protein a series of monoclonal and polyclonal antibodies directed against amyloid-A fibril protein of different species was applied to formalin-fixed paraffin sections using the indirect immunoperoxidase technique. The immunohistochemical results showed a moderate cross-reaction of gazelle amyloid with human, murine, hamster, and canine amyloid-A fibril protein. A strong cross-reaction, however, was found with one of two monoclonal anti-human amyloid-A antibodies and with an antiserum against bovine amyloid-A fibril protein, the amyloid fibril protein of another ungulate. These results demonstrate the presence of amyloid-A fibril protein in the gazelle amyloid and illustrate the diagnostic value of cross-reacting anti-amyloid-A antibodies for the identification of amyloid-A-amyloidosis in species and in individuals in which amyloid has not yet been examined.