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Immunohistochemical Identification of Generalized AA-Amyloidosis in a Mountain Gazelle (Gazella gazella)

Author:
Linke, R. P., Hol, P. R., Geisel, O.
ISSN:
1544-2217
Subject:
Gazella, amyloid, amyloidosis, animal pathology, antiserum, cattle, cross reaction, disease diagnosis, dogs, enzymes, gazelles, hamsters, humans, immunohistochemistry, mice, polyclonal antibodies
Abstract:
Generalized amyloidosis was diagnosed post-mortem in a mountain gazelle (Gazella gazella). To test whether the amyloid deposits consisted of amyloid-A fibril protein a series of monoclonal and polyclonal antibodies directed against amyloid-A fibril protein of different species was applied to formalin-fixed paraffin sections using the indirect immunoperoxidase technique. The immunohistochemical results showed a moderate cross-reaction of gazelle amyloid with human, murine, hamster, and canine amyloid-A fibril protein. A strong cross-reaction, however, was found with one of two monoclonal anti-human amyloid-A antibodies and with an antiserum against bovine amyloid-A fibril protein, the amyloid fibril protein of another ungulate. These results demonstrate the presence of amyloid-A fibril protein in the gazelle amyloid and illustrate the diagnostic value of cross-reacting anti-amyloid-A antibodies for the identification of amyloid-A-amyloidosis in species and in individuals in which amyloid has not yet been examined.
Agid:
6153345