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MOV10 inhibits replication of porcine reproductive and respiratory syndrome virus by retaining viral nucleocapsid protein in the cytoplasm of Marc-145 cells

Zhao, Kuan, Li, Li-Wei, Zhang, Yu-Jiao, Jiang, Yi-Feng, Gao, Fei, Li, Guo-Xin, Yu, Ling-Xue, Zhao, Wen-Ying, Shan, Tong-Ling, Zhou, Yan-Jun, Tong, Guang-Zhi
Biochemical and biophysical research communications 2018 v.504 no.1 pp. 157-163
Porcine reproductive and respiratory syndrome virus, adsorption, control methods, cytoplasm, drugs, fluorescent antibody technique, leukemia, livestock production, nucleocapsid, nucleocapsid proteins, physiological transport, precipitin tests, protein content, swine, virus replication, viruses
Porcine reproductive and respiratory syndrome virus (PRRSV) has been a major threat to global industrial pig farming ever since its emergence in the late 1980s. Identification of sustainable and effective control measures against PRRSV transmission is a pressing problem. The nucleocapsid (N) protein of PRRSV is specifically localized in the cytoplasm and nucleus of virus-infected cells which is important for PRRSV replication. In the current study, a new host restricted factor, Moloney leukemia virus 10-like protein (MOV10), was identified as an inhibitor of PRRSV replication. N protein levels and viral replication were significantly reduced in Marc-145 cells stably overexpressing MOV10 compared with those in wild-type Marc-145 cells. Adsorption experiments revealed that MOV10 did not affect the attachment and internalization of PRRSV. Co-immunoprecipitation and immunofluorescence co-localization analyses showed that MOV10 interacted and co-localized with the PRRSV N protein in the cytoplasm. Notably, MOV10 affected the distribution of N protein in the cytoplasm and nucleus, leading to the retention of N protein in the former. Taken together, these findings demonstrate for the first time that MOV10 inhibits PRRSV replication by restricting the nuclear import of N protein. These observations have great implications for the development of anti-PRRSV drugs and provide new insight into the role of N protein in PRRSV biology.