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Evaluating of quality of rice bran protein concentrate prepared by a combination of isoelectronic precipitation and electrolyzed water treatment
- Watanabe, Masanori, Yamada, Chikako, Maeda, Isamu, Techapun, Charin, Kuntiya, Ampin, Leksawasdi, Noppol, Seesuriyachan, Phisit, Chaiyaso, Thanongsak, Takenaka, Shinji, Shiono, Tadahiko, Nakamura, Kozo, Endo, Shujiro
- Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.99 pp. 262-267
- acid value, allergens, amino acids, corn, electrolyzed water, enzymes, heavy metals, immunoblotting, phosphorus, polyacrylamide gel electrophoresis, protein concentrates, protein content, rice, rice bran, solvents, surfactants, water treatment, wheat
- A combination of isoelectronic precipitation and electrolyzed water treatment (IP-EWT) process was use for simultaneous recovery of protein and phosphorus compounds from heat-stabilized defatted rice bran (HSDFRB). The highest protein content (65.1 w/w%) recovery ratio (over 50%) of protein concentrate were attained by two-stage protein extraction with IP-EWT. Analysis of amino-acid composition demonstrated excellent amino acid value (76.6%) in the protein fraction than those of commercial cereals such as rice (61%), wheat (39%), and corn (31%). In addition, according to SDS-PAGE and immunoblotting analysis, neither rice allergenic protein nor heavy metals were detected in either HSDFRB or protein fractions extracted by IP-EWT. These results suggest that as an eco-friendly process, this combined (IP-EWT) process is suitable for practical recovery of highly concentrated and safe protein from HSDFRB without using enzymes or chemicals such as organic solvents, buffering agents, and surfactants.