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Access to new anticoagulant by sulfation of pectin-like polysaccharides isolated from Opuntia ficus indica cladodes

Chaouch, Mohamed Aymen, Hammi, Khaoula Mkhadmini, Dhahri, Manel, Mansour, Mohamed Ben, Maaroufi, Mounir Raoui, Le Cerf, Didier, Majdoub, Hatem
International journal of biological macromolecules 2018 v.120 pp. 1794-1800
Fourier transform infrared spectroscopy, Opuntia ficus-indica, anticoagulant activity, anticoagulants, arabinose, cladodes, gas chromatography-mass spectrometry, heparin, molecular weight, nuclear magnetic resonance spectroscopy, sulfates, thrombin, thromboplastin
In order to develop new and low cost anticoagulants as potential heparin alternatives, sulfation of a pectin-like polysaccharide from Opuntia ficus indica cladodes using SO3-DMF complex was performed with a significant sulfate content (7%). FTIR and NMR assays indicated that the sulfation reaction had occurred. In addition, GC–MS analyses demonstrated that sulfation was carried out on the arabinose units of native polysaccharide. Moreover, Physico-chemical characterization indicated an evident decrease of the average molecular weight (Mw) and sugar rates after sulfation. Finally, anticoagulant assays demonstrated that the anticoagulant activity was significantly enhanced by the addition of sulfate groups. Thus, sulfated polysaccharides exhibited the most potent anticoagulant activity by prolonging activated partial thromboplastin time (APTT) and thrombin time (TT).