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Two complementary techniques allow detection of Fusarium oxysporum f. sp. radicis-lycopersici in soils from two different tomato-cultivated areas of Chile

Elizondo-Pasten, E., Boix-Ruiz, A., Gomez-Tenorio, M. A., Ruiz-Olmos, C., Marin-Guirao, J. I., Tello-Marquina, J. C., Camacho-Ferre, F.
Acta horticulturae 2018 no.1207 pp. 315-318
Fusarium oxysporum, analytical methods, methyl bromide, organobromine compounds, pathogens, soil, soil fumigants, soil sampling, soil-borne diseases, tomatoes, Chile
Fresh tomato is a highly profitable crop in northern and central Chile. Soil-borne pathogens such as Fusarium oxysporum f. sp. radicis-lycopersici are becoming an increasing threat to the crop. Methyl bromide was widely used as a soil fumigant in these soils, but its use has not been allowed since December 2014. The detection of F. oxysporum f. sp. radicis-lycopersici in infected soils is important in order to develop alternative control strategies to methyl bromide. Two analytical methods have been used to detect the presence of tomato-pathogenic isolates of Fusarium oxysporum: classical dilution plate technique and soil phytopathometry. Both methods were applied to 20 different soil samples, detecting F. oxysporum f. sp. radicis-lycopersici by the dilution technique in 15 of them and by soil phytopathometry in 13 of them. Complementing the two methods, 84% of samples expressed the pathogen. This confirms the presence of the forma specialis in the soils of both cropping areas.