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Engineering CRISPR guide RNA riboswitches for in vivo applications

Author:
Galizi, Roberto, Jaramillo, Alfonso
Source:
Current opinion in biotechnology 2019 v.55 pp. 103-113
ISSN:
0958-1669
Subject:
RNA, biotechnology, gene editing, gene therapy, synthetic biology
Abstract:
CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and multicellular organisms, the tight control of the CRISPR-editing activity remains a major challenge. Here we summarise recent developments of CRISPR and riboswitch technologies and recommend novel functionalised synthetic-gRNA (sgRNA) designs to achieve inducible and spatiotemporal regulation of CRISPR-based genetic editors in response to cellular or extracellular stimuli. We believe that future advances of these tools will have major implications for both basic and applied research, spanning from fundamental genetic studies and synthetic biology to genetic editing and gene therapy.
Agid:
6157328