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Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production

Author:
Shin, Ye Sol, Jeong, Jooyeon, Nguyen, Thu Ha Thi, Kim, Jaoon Young Hwan, Jin, EonSeon, Sim, Sang Jun
Source:
Bioresource technology 2019 v.271 pp. 368-374
ISSN:
0960-8524
Subject:
Chlamydomonas reinhardtii, biodiesel, cell growth, crops, diacylglycerols, fuel production, gene editing, genes, knockout mutants, microalgae, phospholipase A2, triacylglycerols
Abstract:
Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A2 knockout mutants have increased by up to 64.25% (to 80.92 g L−1 d−1). This study can provide crucial information for the biodiesel industry.
Agid:
6157516