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Impact of surface interactions on protein conformation

Faulón Marruecos, David, Schwartz, Daniel K., Kaar, Joel L.
Current opinion in colloid & interface science 2018 v.38 pp. 45-55
bioactive properties, brushes, coatings, computational methodology, films (materials), nanomaterials, physicochemical properties, polymers, protein conformation, proteins, surface interactions, synthetic products
Understanding the behavior of proteins at material interfaces has become increasingly important as the intersection between the biological and synthetic worlds continues to grow. By inducing changes in protein structure, interactions with materials may lead to a loss of function and/or biological activity, as well as undesirable cellular interactions in vivo, including the foreign body reaction to implantable materials. Despite extensive research, the understanding of the mechanisms that lead to protein conformational changes on surfaces remains incomplete. Here, we highlight recent advances in understanding and controlling protein conformation on the surface of synthetic materials, including nanomaterials, polymer films and brushes, metal coatings and particles, and graphitic materials. We also review developments in the use of biophysical and computational methods for characterizing the structure of proteins at solution–solid interfaces. These approaches have provided important new insights into the physico-chemical factors that control protein conformation in near-surface environments, which represents a critical step towards the rational design of biotic–abiotic interfaces in biomedical and biotechnological applications.