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Conquering lignin recalcitrance by Pandoraea sp. B-6 to improve co-solvent pretreatment of corn stover

Zhuo, Shengnan, Peng, Bing, Yan, Xu, Zhang, Kejing, Si, Mengying, Liu, Mingren, Shi, Yan
Process biochemistry 2018 v.75 pp. 187-193
Pandoraea, biomass, cellulose, corn stover, energy, enzymatic hydrolysis, lignin, lignocellulose, sugars, tetrahydrofuran
Chemical pretreatments significantly improve the enzymatic hydrolysis of lignocellulosic biomass, however, lignin, more or less, left in the residue still presents a significant challenge for the sugar yield. To address this challenge, we proposed a pretreatment strategy using bacteria-assisted (tetrahydrofuran) THF–H2O co-solvent (THCB) to enhance enzymatic hydrolysis under mild conditions. Pandoraea sp. B-6 was proved to effectively remove lignin (to 3.7%) in the corn stover (CS) residue after THF–H2O co-solvent (THC) pretreatment. The compositional and structural analysis confirmed that lignin removal contributed to the expose of more cellulose. The sugar yield varied from 129.6 mg/g to 827.2 mg/g after THCB pretreatment under different conditions, which was increased by 42.6%∼2.8% over THC pretreatment (90.8 mg/g∼804.5 mg/g) and 1.5∼9.7 times over the untreated CS (84.8 mg/g). Our founding provides a promising technology for lignocellulosic biomass pretreatment in terms of advanced lignin removal, enzymatic digestibility, and reduced energy consumption.