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Lactobacillus plantarum CQPC06 Activity Prevents Dextran Sulfate Sodium‐Induced Colitis by Regulating the IL‐8 Pathway

Author:
Zhang, Jing, Yi, Ruokun, Qian, Yu, Sun, Peng, Zhao, Xin, Yang, Zhennai
Source:
Journal of food science 2018 v.83 no.10 pp. 2653-2661
ISSN:
0022-1147
Subject:
CXCR1 receptor, CXCR2 receptor, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus plantarum, Western blotting, animal models, animal tissues, bile salts, blood serum, chemokine CCL3, colitis, colon, dextran sulfate, endothelial nitric oxide synthase, gastric acid, genes, inducible nitric oxide synthase, interleukin-1, interleukin-8, lactic acid bacteria, messenger RNA, mice, myeloperoxidase, neuronal nitric oxide synthase, nitric oxide, polypeptides, probiotics, protective effect, protein synthesis, tumor necrosis factor-alpha
Abstract:
Lactobacillus plantarum CQPC06 (LP‐CQPC06) is a newly discovered lactic acid bacterial strain. Here, the beneficial effects of this strain on C57BL/6J mice with dextran sulfate sodium‐induced colitis were investigated. LP‐CQPC06 was more resistant to gastric acid and bile salts than L. delbrueckii subsp. bulgaricus (LB). In the DSS‐induced colitis mouse model, LP‐CQPC06 treatment decreased the colon weight/length ratio and increased the colon length as compared to untreated mice with DSS‐induced colitis. LP‐CQPC06 also reduced the serum levels of interleukin 8 (IL‐8), IL‐1, tumor necrosis factor alpha, and macrophage inflammatory protein‐1 alpha, as well as reducing levels of myeloperoxidase (MPO) and nitric oxide (NO) in the colon tissues of mice with DSS‐induced colitis. In all cases, the effects of LP‐CQPC06 were significantly stronger than those of LB. Quantitative polymerase chain reactions and western blots indicated that LP‐CQPC06 increased the mRNA and protein expression levels of neuronal nitric oxide synthase, endothelial nitric oxide synthase, and nuclear factor of kappa light polypeptide gene enhancer in B‐cells inhibitor alpha, while decreasing the expression levels of inducible nitric oxide synthase, nuclear factor kappa‐beta, C‐X‐C motif chemokine receptor 1 (CXCR1), and CXCR2. Thus, L. plantarum CQPC06 had a good protective effect against colitis in a mouse model via the IL‐8 pathway. Therefore, L. plantarum CQPC06 might have potential uses as a probiotic for colonic protection. PRACTICAL APPLICATION: In this study, a newly discovered lactic acid bacteria was investigated. This bacterial strain had a good prophylactic effect against colitis in a mouse model, and might have potential utility as a probiotic.
Agid:
6158277