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Biodelignification and hydrolysis of rice straw by novel bacteria isolated from wood feeding termite

Author:
Tsegaye, Bahiru, Balomajumder, Chandrajit, Roy, Partha
Source:
3 Biotech 2018 v.8 no.10 pp. 447
ISSN:
2190-572X
Subject:
Bacillus (bacteria), Isoptera, Ochrobactrum, bacteria, biotransformation, carboxymethylcellulose, delignification, digestive system, hydrolysis, lignin, microstructure, reducing sugars, ribosomal RNA, rice straw, sequence analysis, wood, xylan
Abstract:
In this study, two bacterial strains capable of degrading lignin, cellulose, and hemicellulose were isolated from wood feeding termite. The isolates were identified by 16S rRNA gene sequencing. A bacterium Ochrobactrum oryzae BMP03 capable of degrading lignin was isolated on alkali lignin medium and Bacillus sp. BMP01 strain capable of degrading cellulose and hemicellulose were isolated on carboxymethyl cellulose and xylan media. The efficiency of bacterial degradation was studied by evaluating the composition of rice straw both before and after degradation. The appearance of new cellulose bands at 1382, 1276, 1200, and 871 cm⁻¹, and the absence of former lignin bands at 1726, 1307, and 1246 cm⁻¹ was observed after biodelignification. This was further confirmed by the formation of channeling and layering of the microstructure of biodelignified rice straw observed under electron microscope. Maximum lignin removal was achieved in separate biodelignification and hydrolysis process after the 14th day of treatment by Ochrobactrum oryzae BMP03 (53.74% lignin removal). Hydrolysis of the biodelignified rice straw released 69.96% of total reducing sugars after the 14th day hydrolysis by Bacillus sp. BMP01. In simultaneous delignification and hydrolysis process, about 58.67% of total reducing sugars were obtained after the 13th day of biotreatment. Separate delignification and hydrolysis process were found to be effective in lignin removal and sugar released than the simultaneous process. The bacteria, Bacillus sp. BMP01, has the ability to degrade hemicellulose and cellulose simultaneously. Overall, these results demonstrate that the possibility of rice straw bioconversion into reducing sugars by bacteria from termite gut.
Agid:
6159086