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Effects of Icaritin on the physiological activities of esophageal cancer stem cells

Author:
Han, Songchen, Gou, Yunjiu, Jin, Dacheng, Ma, Jilong, Chen, Meng, Dong, Xinchun
Source:
Biochemical and biophysical research communications 2018 v.504 no.4 pp. 792-796
ISSN:
0006-291X
Subject:
Western blotting, apoptosis, dose response, drugs, esophageal neoplasms, flow cytometry, neoplasm cells, proteins, signal transduction, stem cells, therapeutics
Abstract:
Icaritin is a compound extracted from herb, recent study have found it is able to influence the activity of various types of cancer. Our aim was to investigate the effects of Icaritin on the physiological activities of esophageal cancer stem cells (CSCs). In this study, esophageal cancer cells were cultured and CD133 positive esophageal CSCs were sorted by flow cytometry. Changes in the physiological activity of esophageal CSCs following treatment with different concentrations of Icaritin (0, 12.5, 25, 50, and 100 μmol/L) were evaluated. The CCK-8 method and Transwell assay were used to determine the effects of Icaritin on the proliferation, migration, and invasion of esophageal CSCs. Flow cytometery was used to investigate its effect on the apoptosis of CSCs. The effect of Icaritin on the expression of proteins in Wnt and Hedgehog signaling pathways were determined using western blot test. Consequently, Icaritin inhibited the proliferation, migration, and invasion of esophageal CSCs in a dose-dependent manner. It promoted cell apoptosis, and influenced the levels of proteins in Wnt and Hedgehog signaling pathways. It may act as a promising drug in the therapy of esophageal cancer.
Agid:
6159177