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Assessment of the subcutaneous degradation process of insoluble hyaluronic acid in rats

Author:
Uemura, Akiko, Takiguchi, Masahito, Funakoshi, Kengo, Noishiki, Yasuharu, Ogawa, Shingo, Tanaka, Ryou
Source:
Biochemical and biophysical research communications 2018 v.505 no.2 pp. 511-515
ISSN:
0006-291X
Subject:
adhesion, females, hyaluronic acid, rats
Abstract:
Insoluble hyaluronic acid (IHA) may prevent adhesions by forming a physical barrier during the period when postoperative adhesions form. This study was performed to verify the changes that a solid IHA membrane undergoes as it is degraded in vivo, and to ascertain the swelling rate of IHA required for it to function as a physical barrier during the postoperative adhesion formation period. Nine female WI rats weighing 300–400 g were used. Discs 8 mm in diameter were cut out of dry IHA membranes made of IHA with a swelling rate (wet weight/dry weight) of either 2.47 (high-swelling IHA) or 1.94 (low-swelling IHA). They were placed in saline to swell and then washed with saline before subcutaneous implantation in four pockets in each rat. The high-swelling IHA started to degrade more rapidly than the low-swelling IHA. There was no evidence of degradation of the low-swelling IHA until day 7, but once it had started, the speed of degradation tended to be similar to that of the high-swelling IHA. The present results showed that, when IHA is implanted subcutaneously in rats, it is degraded over time in a phased process. The swelling rate required for the use of IHA as a postoperative adhesion barrier was also suggested.
Agid:
6159229