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A biotechnological approach to immunotherapy: Antivenom against Crotalus durissus cascavella snake venom produced from biodegradable nanoparticles
- Gláucia-Silva, Fiamma, Torres-Rêgo, Manoela, Rocha Soares, Karla Samara, Damasceno, Igor Zumba, Tambourgi, Denise Vilarinho, Silva-Júnior, Arnóbio Antônio da, Fernandes-Pedrosa, Matheus de Freitas
- International journal of biological macromolecules 2018 v.120 pp. 1917-1924
- Crotalus durissus, Fourier transform infrared spectroscopy, aluminum hydroxide, animal models, antibodies, antivenoms, biodegradability, chitosan, death, gelation, immunization, mice, nanoparticles, proteins, snake bites, snake venoms, snakes, tropical diseases, vaccines, Brazil
- Snakebite envenoming is a tropical disease neglected worldwide. In Brazil, the Crotalus durissus cascavella (CDC) snake belongs to a genus with venom of highest lethality. A search for new immunoadjuvants aimed to expand the therapeutic alternatives to improve vaccines and antivenom. This approach proposed to produce small and narrow-sized cationic CDC venom-loaded chitosan nanoparticles (CHNP) able to induce antibody response against the CDC venom. The ionic gelation method induced the formation of stable and slightly smooth spherical nanoparticles (<160 nm) with protein loading efficiency superior to 90%. The interactions between venom proteins and CHNP assessed using FT-IR spectroscopy corroborated with the in vitro release behavior of proteins from nanoparticles. Finally, the immunization animal model using BALB/c mice demonstrated the higher effectiveness of CDC venom-loaded CHNP compared to aluminum hydroxide, a conventional immunoadjuvant. Thus, CHNPs loaded with CDC venom exhibited a promising biotechnological approach to immunotherapy.