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Specific discrimination of gram-positive bacteria and direct visualization of its infection towards mammalian cells by a DPAN-based AIEgen

Hu, Rong, Zhou, Fan, Zhou, Taotao, Shen, Jianlei, Wang, Zhiming, Zhao, Zujin, Qin, Anjun, Tang, Ben Zhong
Biomaterials 2018 v.187 pp. 47-54
Gram-positive bacteria, fluorescence, fungi, mammals, morbidity, mortality, public health, therapeutics
The infections caused by gram-positive bacteria (G⁺) is a major threat to public health due to their high morbidity and mortality, and the identification of G⁺ with high sensitivity and selectivity is urgently desirable. In this paper, a probe with aggregation-induced emission (AIE) characteristic is developed and used to discriminate G⁺ from other bacteria and fungus with excellent selectivity. Thanks for its AIE feature, this probe shows intense fluorescence when interacted with G⁺, and the signal can last for 24 h after incubating with S. aureus, which is benefit for long term tracing of translocation process with high contrast. Moreover, this probe can be used to visualize the infection processes of G⁺ towards mammalian cells, which provides valuable information for understanding the therapeutic mechanism.