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Structural elucidation and anti-osteoporosis activities of polysaccharides obtained from Curculigo orchioides

Author:
Wang, Xueqian, Zhang, Mengliu, Zhang, Dawei, Wang, Xinluan, Cao, Huijuan, Zhang, Qian, Yan, Chunyan
Source:
Carbohydrate polymers 2019 v.203 pp. 292-301
ISSN:
0144-8617
Subject:
Curculigo orchioides, Oriental traditional medicine, bone formation, bones, estradiol, mineralization, ovariectomy, polysaccharides, rats, rhizomes, tendons
Abstract:
Curculigo orchioides, is a traditional Chinese medicine, is used in strengthening tendons and bones. We evaluated the anti-osteoporosis activity of the crude polysaccharide (CO70) isolated from the rhizomes of C. orchioides in ovariectomized rats. CO70 showed excellent anti-osteoporosis activity comparable to that of 17β-estradiol. To explore the constituents responsible for the anti-osteoporosis activity of CO70, a novel homogeneous heteropolysaccharide, COP70-3, was isolated and purified from CO70. COP70-3 has a main backbone chain of (1→5)-linked α-L-Araf, (1,3→5)-linked α-L-Araf, (1→6)-linked β-D-Galp, (1→4)-linked β-D-Manp, (1,2→5)-linked α-L-Araf, (1→3)-linked β-L-Rhap, (1, 3→6)-linked β-D-Manp, (1→3)-linked α-D-GalpA, (1,3→6)-linked β-D-Galp and (1→6)-linked α-D-Glcp residues. Furthermore, 1.87 nM COP70-3 obviously promoted the differentiation of MC3T3-E1 cells, while 0.94 and 1.87 nM COP70-3 significantly improved the osteogenic mineralization rate. These data indicate that COP70-3 has favorable anti-osteoporosis activity in vitro.
Agid:
6160067