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Cyclic GMP mediates abscisic acid-stimulated isoflavone synthesis in soybean sprouts

Jiao, Caifeng, Gu, Zhenxin
Food chemistry 2019 v.275 pp. 439-445
G-proteins, abscisic acid, bean sprouts, biosynthesis, calreticulin, cyclic GMP, gene expression, gene expression regulation, isoflavones, naringenin-chalcone synthase, protein kinases, protein synthesis, soybeans, ultraviolet radiation
The influences of abscisic acid (ABA)-guanosine 3′,5′-cyclic monophosphate (cGMP) on UV-B treatment-stimulated isoflavone synthesis in soybean sprouts was explored. It turned out that ABA, with cGMP, up-regulated gene expression and activity of chalcone synthase (CHS) and isoflavone synthase (IFS), and subsequently induced isoflavone biosynthesis under UV-B treatment. Furthermore, data obtained from the isobaric tags for relative and absolute quantification (iTRAQ) analysis showed that there were two core components in ABA response: SNF1-related protein kinase (SnRK) and type 2C protein phosphatase (PP2C), were up and down regulated after UV-B treatment, respectively. UV-B exposure stimulated increment in guanine nucleotide-binding protein and calreticulin expression. Additionally, CHS and IFS protein expression were up regulated under UV-B stress. Overall, UV-B-induced ABA resulted in PP2C inhibition and SnRK2 activation, and up-regulated CHS and IFS expression, leading to enhancement of isoflavone accumulation. cGMP and calreticulin as downstream messengers, mediated ABA-stimulated isoflavone biosynthesis after UV-B exposure.