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Population diversity of Diaphorina citri (Hemiptera: Liviidae) in China based on whole mitochondrial genome sequences

Wu, Fengnian, Jiang, Hongyan, Beattie, G Andrew C, Holford, Paul, Chen, Jianchi, Wallis, Christopher M, Zheng, Zheng, Deng, Xiaoling, Cen, Yijing
Pest management science 2018 v.74 no.11 pp. 2569-2577
Candidatus Liberibacter asiaticus, Citrus, Diaphorina citri, genes, greening disease, mitochondria, mitochondrial genome, pest management, phylogeny, single nucleotide polymorphism, China
BACKGROUND: Diaphorina citri (Asian citrus psyllid; ACP) transmits ‘Candidatus Liberibacter asiaticus’ associated with citrus Huanglongbing (HLB). ACP has been reported in 11 provinces/regions in China, yet its population diversity remains unclear. In this study, we evaluated ACP population diversity in China using representative whole mitochondrial genome (mitogenome) sequences. Additional mitogenome sequences outside China were also acquired and evaluated. RESULTS: The sizes of the 27 ACP mitogenome sequences ranged from 14 986 to 15 030 bp. Along with three previously published mitogenome sequences, the 30 sequences formed three major mitochondrial groups (MGs): MG1, present in southwestern China and occurring at elevations above 1000 m; MG2, present in southeastern China and Southeast Asia (Cambodia, Indonesia, Malaysia, and Vietnam) and occurring at elevations below 180 m; and MG3, present in the USA and Pakistan. Single nucleotide polymorphisms in five genes (cox2, atp8, nad3, nad1 and rrnL) contributed mostly in the ACP diversity. Among these genes, rrnL had the most variation. CONCLUSION: Mitogenome sequences analyses revealed two major phylogenetic groups of ACP present in China as well as a possible unique group present currently in Pakistan and the USA. The information could have significant implications for current ACP control and HLB management. © 2018 Society of Chemical Industry