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Adjuvant effects on protection and immune response of Japanese flounder immunized by the formalin-killed cells of Edwardsiella tarda
- Kondo, Hidehiro, Chung, Seangmin, Hirosawa, Eriko, Hirono, Ikuo
- Fish & shellfish immunology 2019 v.84 pp. 120-123
- Edwardsiella tarda, Paralichthys olivaceus, adjuvants, antibodies, flounder, gene expression, gene expression regulation, immune response, interferon-gamma, interleukin-12, messenger RNA, mineral oil, shellfish, signal transduction
- We evaluated the effects of Freund's adjuvants (FCA/FIA) on protection and immune response of Japanese flounder Paralichthys olivaceus immunized by the formalin-killed cell (FKC) of Edwardsiella tarda. Combination of FKC and FCA/FIA did not confer protection against the challenge, while they significantly induced higher antibody titers than that of FKC only. The suppression of FKC-dependent induction of interferon γ (IFNγ) mRNA levels by FCA/FIA was observed by gene expression profiling. Similarly, interleukin (IL)-12 p35 mRNA levels were not detected after FKC+FCA or +FIA. These results suggest that the mineral oil in Freund's adjuvants might suppress the signaling pathway(s) that induce IFNγ and IL-12 gene expression.