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Molecular cloning, characterization and mRNA expression of duck interleukin-17F
- Kim, Woo H., Fernandez, Cherry P., Diaz, Joyce Anne R., Jeong, Jipseol, Kim, Suk, Lillehoj, Hyun S., Chang, Hong H., Min, Wongi
- Veterinary immunology and immunopathology 2015 v.164 no.3-4 pp. 194
- Salmonella Typhimurium, chickens, complementary DNA, concanavalin A, cysteine, disease models, ducks, fibroblasts, gene expression, glycosylation, homeostasis, interleukin-17, interleukin-1beta, interleukin-6, interleukin-8, intestines, kidneys, liver, lymphocytes, mammals, messenger RNA, molecular cloning, nucleotide sequences, salmonellosis, sequence analysis, signal peptide, spleen, thymus gland
- Interleukin-17F (IL-17F) is a proinflammatory cytokine that plays an important role in gut homeostasis. A full-length duck IL-17F (duIL-17F) cDNA with a 510-bp coding region was identified in ConA-activated splenic lymphocytes. duIL-17F is predicted to encode 166 amino acids, including a 26-amino acid signal peptide, a single N-linked glycosylation site, and six cysteine residues that are conserved in mammalian IL-17. duIL-17F shares 77.5% amino acid sequence identity with chicken IL-17F (chIL-17F), 37–46% with corresponding mammalian homologues, and 53.5% with the previously described duck IL-17A (duIL-17A). The duIL-17F transcripts were expressed in a wide range of untreated tissues; levels were highest in the liver and moderate in the thymus, bursa, kidney, and intestinal tissues. Expression levels of duIL-17F transcript were slightly up-regulated in ConA- and LPS-activated splenic lymphocytes but not in poly I:C stimulated cells. duIL-17F forms heterodimers with duIL-17A. Recombinant duIL-17F, like duIL-17A, induced IL-1β, IL-6, and IL-8 expression in duck embryonic fibroblasts (DEFs). duIL-17A, but not duIL-17F expression, was significantly up-regulated in the liver and spleen of Salmonella Typhimurium-infected ducks. Further analysis of the contributions of IL-17F to different Salmonella spp. or other disease models will be required to expand our understanding of its biological functions.