Jump to Main Content
Mycoplasma Iowae Species-Specific DNA Probe
- Zhao, Shaohua, Yamamoto, Richard
- DNA probes, Escherichia coli, Mycoplasma gallisepticum, Mycoplasma iowae, Mycoplasma synoviae, birds, genomic libraries, nucleic acid hybridization, plasmids
- A Mycoplasma iowae (MI) species-specific DNA probe (designated pMI-2) of 6.0 kbp (kilobase pairs) was isolated from an MI strain I-695 genomic library prepared in plasmid pUC8 and Escherichia coli strain JM83. When labeled with [³²]P by nick translation, the probe hybridized in dot blot assays with 6 reference strains and 8 field isolates of MI but not with 16 other known species of avian mycoplasmas. The pMI-2 probe detected a minimum of 1.5 ng of MI strain I-695 chromosomal DNA. Under identical conditions of hybridization, the probe did not hybridize with a high concentration (200 ng) of M. gallisepticum or M. synoviae chromosomal DNA.