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A combined small RNA and transcriptome sequencing analysis reveal regulatory roles of miRNAs during anther development of Upland cotton carrying cytoplasmic male sterile Gossypium harknessii (D2) cytoplasm

Author:
Zhang, Bingbing, Zhang, Xuexian, Liu, Guoyuan, Guo, Liping, Qi, Tingxiang, Zhang, Meng, Li, Xue, Wang, Hailin, Tang, Huini, Qiao, Xiuqin, Pei, Wenfeng, Shahzad, Kashif, Xing, Chaozhu, Zhang, Jinfa, Wu, Jianyong
Source:
BMC plant biology 2018 v.18 no.1 pp. 242
ISSN:
1471-2229
Subject:
Gossypium hirsutum, breeding, buds, cotton, cytoplasmic male sterility, gene expression regulation, genes, hybrids, meiosis, microRNA, mitochondria, mitochondrial genome, sequence diversity, transcriptomics
Abstract:
BACKGROUND: Cytoplasmic male sterility (CMS) in flowering plants is usually caused by incompatibility between mitochondrial and nuclear genomes, and can be restored by nuclear genes known as restorer-of-fertility (Rf). Although the CMS/Rf system is useful and convenient for economic production of commercial hybrid seed, the molecular mechanisms of CMS occurrence and fertility restoration in cotton are unclear. RESULTS: Here, a combined small RNA and transcriptome sequencing analysis was performed on floral buds at the meiosis stage in three-line hybrid cotton system, and differentially expressed microRNAs (DEMs) and their target genes were identified and further analyzed for a possible involvement in CMS and fertility restoration. Totally 10 and 30 differentially expressed miRNA-target gene pairs were identified in A-B and A-R comparison group, respectively. A putative regulatory network of CMS occurrence and fertility restoration-related miRNA-target pairs during anther development were then constructed. The RLM-RACE analysis showed that gra-miR7505b regulates a PPR gene (Gh_D05G3392) by cleaving precisely at the 643 nt and 748 nt sites. The further analysis indicated that the sequence variation in the binding regions of Gh_D05G3392 and Gh_D05G3356 may cause a lower cleavage efficiency of the PPR genes by miR7505b and miR7505 in R line, respectively, leading to the up-regulation of the PPR genes and fertility restoration. These results have established their genetic involvement in fertility restoration in the CMS-D2 system. CONCLUSION: Our combined miRNA and transcriptome analysis in three-line hybrid cotton system provides new insights into the molecular mechanisms of CMS occurrence and fertility restoration, which will contribute to further hybrid breeding in cotton.
Agid:
6165853