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Phycocyanin Reduces Proliferation of Melanoma Cells through Downregulating GRB2/ERK Signaling

Hao, Shuai, Li, Shuang, Wang, Jing, Zhao, Lei, Zhang, Chan, Huang, Weiwei, Wang, Chengtao
Journal of agricultural and food chemistry 2018 v.66 no.41 pp. 10921-10929
food additives, functional foods, isotope labeling, melanoma, phenotype, phycocyanin, protein synthesis, proteomics, radionuclides, sulfur, therapeutics
As a type of functional food additive, phycocyanin is shown to have a potential antineoplastic property. However, its underlying anticancer mechanism in melanoma cells remains unknown. We previously reported a ³⁵S in vivo/vitro labeling analysis for dynamic proteomic (SiLAD) technology. It could exclusively detect protein synthesis rates via pulse labeling of newly expressed proteins by ³⁵S, providing a high time-resolution method for analysis of protein variations. In the present study, we performed a time course analysis in A375 melanoma cells after phycocyanin treatment using SiLAD. Protein expression velocities were specifically visualized and their regulation modes were dynamically traced. Strikingly, novel protein synthesis patterns were discovered in the early phase of phycocyanin treatment, suggesting a possible mechanism of phycocyanin regulation. Furthermore, network analysis and phenotype experiments demonstrated that GRB2-ERK1/2 pathway was involved in phycocyanin-mediated regulation process and responsible for the proliferation suppression of melanoma cell, which could be a therapeutic target for malignant melanoma.