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Immune Activation of RAW264.7 Macrophages by Low Molecular Weight Fucoidan Extracted from New Zealand Undaria pinnatifida

Bi, Decheng, Yu, Boming, Han, Qingguo, Lu, Jun, White, William Lindsey, Lai, Qiuxian, Cai, Nan, Luo, Wenqi, Gu, Liang, Li, Sheng, Xu, Hong, Hu, Zhangli, Nie, Shaoping, Xu, Xu
Journal of agricultural and food chemistry 2018 v.66 no.41 pp. 10721-10728
Undaria pinnatifida, bioactive properties, fucoidan, immunomodulation, inducible nitric oxide synthase, interleukin-6, macrophages, mitogen-activated protein kinase, molecular weight, nitric oxide, secretion, signal transduction, transcription factor NF-kappa B, tumor necrosis factor-alpha, New Zealand
Fucoidan, a sulfated polysaccharide extracted from brown seaweeds, has been shown to possess various bioactivities. In particular, low molecular weight fucoidan (LMWF) has been shown to have better bioactivities. In this study, a LMWF (<10 kDa) was extracted from New Zealand Undaria pinnatifida and investigated for its immune modulation effects. LMWF at a concentration range from 1 to 50 μg/mL exerted an effective immune activation in RAW264.7 macrophages. LMWF treatment promoted significant NO release, iNOS expression, and TNF-α and IL-6 secretion in a concentration-dependent manner. It also significantly stimulated the activation of NF-κB and MAPK signaling pathways, and specific inhibitors of NF-κB and MAPK pathways diminished the stimulation, confirming the activation pathways. These results indicate that LMWF possesses potential health benefits through immune-stimulation, which may lead to future pharmaceutical development.