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Immune Activation of RAW264.7 Macrophages by Low Molecular Weight Fucoidan Extracted from New Zealand Undaria pinnatifida

Author:
Bi, Decheng, Yu, Boming, Han, Qingguo, Lu, Jun, White, William Lindsey, Lai, Qiuxian, Cai, Nan, Luo, Wenqi, Gu, Liang, Li, Sheng, Xu, Hong, Hu, Zhangli, Nie, Shaoping, Xu, Xu
Source:
Journal of agricultural and food chemistry 2018 v.66 no.41 pp. 10721-10728
ISSN:
1520-5118
Subject:
Undaria pinnatifida, bioactive properties, fucoidan, immunomodulation, inducible nitric oxide synthase, interleukin-6, macrophages, mitogen-activated protein kinase, molecular weight, nitric oxide, secretion, signal transduction, transcription factor NF-kappa B, tumor necrosis factor-alpha, New Zealand
Abstract:
Fucoidan, a sulfated polysaccharide extracted from brown seaweeds, has been shown to possess various bioactivities. In particular, low molecular weight fucoidan (LMWF) has been shown to have better bioactivities. In this study, a LMWF (<10 kDa) was extracted from New Zealand Undaria pinnatifida and investigated for its immune modulation effects. LMWF at a concentration range from 1 to 50 μg/mL exerted an effective immune activation in RAW264.7 macrophages. LMWF treatment promoted significant NO release, iNOS expression, and TNF-α and IL-6 secretion in a concentration-dependent manner. It also significantly stimulated the activation of NF-κB and MAPK signaling pathways, and specific inhibitors of NF-κB and MAPK pathways diminished the stimulation, confirming the activation pathways. These results indicate that LMWF possesses potential health benefits through immune-stimulation, which may lead to future pharmaceutical development.
Agid:
6166606