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Toxicity impacts of chemically and biologically synthesized CuO nanoparticles on cell suspension cultures of Nicotiana tabacum

Author:
Mahjouri, Sepideh, Movafeghi, Ali, Divband, Baharak, Kosari-Nasab, Morteza
Source:
Plant cell, tissue, and organ culture 2018 v.135 no.2 pp. 223-234
ISSN:
0167-6857
Subject:
Nicotiana tabacum, cell biology, cell suspension culture, copper nanoparticles, cupric oxide, dose response, enzyme activity, malondialdehyde, plant extracts, polymers, pyrolysis, quality of life, risk, tobacco, toxicity, viability
Abstract:
Nanotechnology has quite a lot of applications in various fields of industrial sectors like food and agriculture. Although nanotechnology can improve the quality of life, its possible associated risks should be assessed. Here copper oxide nanoparticles (CuO NPs) were synthesized by chemical (polymer pyrolysis) and biological (green) methods with an average size of 30 and 44 nm, respectively. Afterwards, a cell biology approach was applied to evaluate the toxic effects of chemically and biologically synthesized CuO nanoparticles on tobacco cell suspension cultures. Both types of CuO nanoparticles significantly dropped the viability of the cells in a dose and time dependent manner. Accordingly, tobacco cells were found to increase the activity of antioxidant enzymes after 48 h of exposure to nanoparticles. The production of reactive oxygen species (ROS) and malondialdehyde (MDA) in a dose dependent manner was also observed. Assessment of the toxicity of CuO NPs revealed that chemically synthesized NPs were more toxic than biologically synthesized ones. It can be concluded that the organic components of the plant extract as capping agents that remain on the surface of green synthesized CuO NPs may reduce their toxicity effects.
Agid:
6167114