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Effect of 1‐ and 2‐Month High‐Dose Alpha‐Linolenic Acid Treatment on 13C‐Labeled Alpha‐Linolenic Acid Incorporation and Conversion in Healthy Subjects

Pignitter, Marc, Lindenmeier, Michael, Andersen, Gaby, Herrfurth, Cornelia, Beermann, Christopher, Schmitt, Joachim J., Feussner, Ivo, Fulda, Martin, Somoza, Veronika
Molecular nutrition & food research 2018 v.62 no.20 pp. e1800271
acid treatment, alpha-linolenic acid, carbon, erythrocytes, food intake, isotope labeling, linseed oil, low density lipoprotein, models, phospholipids, stable isotopes
SCOPE: The study aims at identifying 1) the most sensitive compartment among plasma phospholipids, erythrocytes, and LDL for studying alpha‐linolenic acid (ALA) conversion, and 2) whether ALA incorporation and conversion is saturable after administration of ¹³C‐labeled ALA‐rich linseed oil (LO). The effect of a daily intake of 7 g nonlabeled LO (>43% w/w ALA) for 1 month after bolus administration of 7 g ¹³C‐labeled LO on day 1, and for 2 months after bolus administration of 7 g ¹³C‐labeled LO on day 1 and day 29 on ¹³C‐ALA incorporation and conversion into its higher homologs is investigated in healthy volunteers. METHODS AND RESULTS: Incorporation and conversion of LO‐derived ¹³C‐labeled ALA is quantified by applying compartmental modeling. After bolus administration, a fractional conversion of approximately 30% from ¹³C‐ALA to ¹³C‐DHA is calculated as reflected by the LDL compartment. Treatment with LO for 8 weeks induces a mean reduction of ¹³C‐ALA conversion to ¹³C‐DHA by 48% as reflected by the LDL compartment, and a mean reduction of the ¹³C‐ALA incorporation into LDL by 46%. CONCLUSION: A 2‐month dietary intake of a high dose of LO is sufficient to reach saturation of ALA incorporation into LDL particles, which are responsible for ALA distribution in the body.