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Determination of Sulfonamides in Chicken Meat by Magnetic Molecularly Imprinted Polymer Coupled to HPLC-UV

Karimi, Mohammad, Aboufazeli, Forouzan, Zhad, Hamid Reza Lotfi Zadeh, Sadeghi, Omid, Najafi, Ezzatollah
Food analytical methods 2014 v.7 no.1 pp. 73-80
Fourier transform infrared spectroscopy, X-ray diffraction, adsorption, chicken meat, desorption, detection limit, methanol, nanoparticles, scanning electron microscopes, sulfonamides, thermal analysis
In this work, Fe3O4 magnetic nanoparticles were synthesized and modified by a molecularly imprinted polymer for easy and selective extraction and determination of sulfonamides in chicken meat samples. Imprinted polymer magnetic nanoparticles were characterized by Fourier transform infrared spectroscopy, X-ray diffraction pattern, thermal analysis, and scanning electron microscope micrograph. The template was removed by methanol elution. The effective parameters on extraction and determination of sulfonamides on the sorbent such as eluent type, extraction solvent, and adsorption and desorption times were optimized. Sulfonamide separation and determination were performed by high-performance liquid chromatography-UV. The linear ranges for sulfonamides were 0.5-150 μg/L and the limits of detection were 0.1-0.5 μg/L. Relative standard deviations were within 4.3-5.4 %. The method showed good selectivity for extraction of sulfonamides in real samples. ©Springer Science+Business Media New York 2013.