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New paper-based microfluidic tools for the analysis of blood serum protein and creatinine built via aerosolized deposition of polycaprolactone

Heist, Christopher A., Bandara, Gayan C., Bemis, David J., Pommerenck, Joel C., Remcho, Vincent T.
Analytical methods 2018 v.10 no.25 pp. 2994-3000
blood proteins, blood serum, colorimetry, creatinine, hydrophilicity, hydrophobicity
This article describes a low-cost method for rapid fabrication of paper-based microfluidic devices using an aerosolized polymeric solution and substrates masked with painter's tape. This approach requires only a few simple tools and uses low-cost supplies to achieve fully functional microfluidic paper-based analytical devices. The method is capable of producing devices with minimum hydrophilic channel widths of 482 ± 4 μm, with the channel height being dictated by the substrate thickness. Complete hydrophobic barriers can be achieved with widths of only 257 ± 2 μm. This technique enables two dimensional (2D) fluidic pattern fabrication on a single membrane while complex three dimensional (3D) fluidic pattern fabrication is possible by simply incorporating a stack and lamination step. Devices built using this approach were applied in two clinical diagnostic applications: quantitative colorimetric assays for protein and for creatinine.