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Application of succulent plant leaves for Agrobacterium infiltration-mediated protein production

Author:
Richard W. Jones
Source:
Journal of microbiological methods 2016 v.120 pp. 65-67
ISSN:
0167-7012
Subject:
Agrobacterium, Hylotelephium telephium, Kalanchoe blossfeldiana, cacti and succulents, cell walls, enzymes, genetic transformation, leaves, protein synthesis
Abstract:
When expressing plant cell wall degrading enzymes in the widely used tobacco (Nicotiana benthamiana) after Agrobacterium infiltration, difficulties arise due to the thin leaf structure. Thick leaved succulents, Kalanchoe blossfeldiana and Hylotelephium telephium, were tested as alternatives. A xyloglucanase, as well as a xyloglucanase inhibitor protein was successfully produced.
Agid:
61737
Handle:
10113/61737