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Application of succulent plant leaves for Agrobacterium infiltration-mediated protein production
- Richard W. Jones
- Journal of microbiological methods 2016 v.120 pp. 65-67
- Agrobacterium, Hylotelephium telephium, Kalanchoe blossfeldiana, cacti and succulents, cell walls, enzymes, genetic transformation, leaves, protein synthesis
- When expressing plant cell wall degrading enzymes in the widely used tobacco (Nicotiana benthamiana) after Agrobacterium infiltration, difficulties arise due to the thin leaf structure. Thick leaved succulents, Kalanchoe blossfeldiana and Hylotelephium telephium, were tested as alternatives. A xyloglucanase, as well as a xyloglucanase inhibitor protein was successfully produced.