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2-Oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2

Author:
Abboud, Martine I., McAllister, Tom E., Leung, Ivanhoe K. H., Chowdhury, Rasheduzzaman, Jorgensen, Christian, Domene, Carmen, Mecinović, Jasmin, Lippl, Kerstin, Hancock, Rebecca L., Hopkinson, Richard J., Kawamura, Akane, Claridge, Timothy D. W., Schofield, Christopher J.
Source:
Chemical communications 2018 v.54 no.25 pp. 3130-3133
ISSN:
1364-548X
Subject:
animals, hydroxylation, hypoxia, iron, procollagen-proline dioxygenase
Abstract:
Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(ii)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ∼50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding.
Agid:
6174644