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The complete mitochondrial genome of the hybrid of Acipenser dabryanus (♀) × A. schrenckii (♂) and its phylogeny

Author:
Zou, Yuanchao, Zhang, Junying, Wen, Zhengyong, Qin, Chuanjie, Wang, Yongming, Li, Rui, Qi, Zemin
Source:
Conservation genetics resources 2018 v.10 no.4 pp. 881-884
ISSN:
1877-7252
Subject:
Acipenser dabryanus, Acipenser schrenckii, Acipenser sinensis, genes, genetic markers, high-throughput nucleotide sequencing, hybrids, inheritance (genetics), loci, mitochondria, mitochondrial genome, parents, phylogeny, population genetics, replication origin, ribosomal RNA, species identification, statistical analysis, sturgeon, transfer RNA
Abstract:
The complete mitochondrial genome sequence of the hybrid of Acipenser dabryanus (♀) × A. schrenckii (♂) was first determined by using the next-generation sequencing in this study. The circular mitochondrial genome was 16,439 bp in length, which contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, one displacement loop locus and an origin of replication on the light-strand, showed a typical vertebrate pattern. All of the genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall nucleotide composition was 30.19% A, 23.74% T, 29.65% C, 16.42% G, with 53.93% AT, respectively. Compared with the complete mitogenome of the parents, the results indicated that the mitochondria of hybrid sturgeon could be consistent with a maternal inheritance. Phylogenetic analyses using concatenated nucleotide sequences of the 13 protein-coding genes with two different methods (maximum likelihood and neighbor-joining analysis) both highly supported that A. dabryanus (♀) × A. schrenckii (♂) showed a close relationship with A. dabryanus and A. sinensis. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.
Agid:
6175564