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The complete mitochondrial genome of the hybrid of Acipenser dabryanus (♀) × A. schrenckii (♂) and its phylogeny

Zou, Yuanchao, Zhang, Junying, Wen, Zhengyong, Qin, Chuanjie, Wang, Yongming, Li, Rui, Qi, Zemin
Conservation genetics resources 2018 v.10 no.4 pp. 881-884
Acipenser dabryanus, Acipenser schrenckii, Acipenser sinensis, genes, genetic markers, high-throughput nucleotide sequencing, hybrids, inheritance (genetics), loci, mitochondria, mitochondrial genome, parents, phylogeny, population genetics, replication origin, ribosomal RNA, species identification, statistical analysis, sturgeon, transfer RNA
The complete mitochondrial genome sequence of the hybrid of Acipenser dabryanus (♀) × A. schrenckii (♂) was first determined by using the next-generation sequencing in this study. The circular mitochondrial genome was 16,439 bp in length, which contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, one displacement loop locus and an origin of replication on the light-strand, showed a typical vertebrate pattern. All of the genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall nucleotide composition was 30.19% A, 23.74% T, 29.65% C, 16.42% G, with 53.93% AT, respectively. Compared with the complete mitogenome of the parents, the results indicated that the mitochondria of hybrid sturgeon could be consistent with a maternal inheritance. Phylogenetic analyses using concatenated nucleotide sequences of the 13 protein-coding genes with two different methods (maximum likelihood and neighbor-joining analysis) both highly supported that A. dabryanus (♀) × A. schrenckii (♂) showed a close relationship with A. dabryanus and A. sinensis. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.