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Protective effects of a Ganoderma atrum polysaccharide against acrylamide induced oxidative damage via a mitochondria mediated intrinsic apoptotic pathway in IEC-6 cells

Author:
Jiang, Guoyong, Zhang, Lulu, Wang, Hui, Chen, Qian, Wu, Xiaolin, Yan, Xiaoli, Chen, Yi, Xie, Mingyong
Source:
Food & function 2018 v.9 no.2 pp. 1133-1143
ISSN:
2042-650X
Subject:
Ganoderma, acetylcysteine, acrylamides, antioxidants, apoptosis, caspase-3, cytochrome c, glutathione peroxidase, intestines, malondialdehyde, membrane potential, mitochondria, mitochondrial membrane, oxidative stress, polysaccharides, protective effect, superoxide dismutase, toxicity
Abstract:
The preventive role of a purified Ganoderma atrum polysaccharide PSG-1-F₂ as a new dietary antioxidant against the intestinal toxicity of acrylamide (ACR) was investigated in vitro. Our results showed that ACR could induce oxidative stress in IEC-6 cells by the overproduction of reactive oxygen species (ROS) and malondialdehyde (MDA), and as well as the reduction in the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). In addition, the induction of a mitochondria-mediated intrinsic apoptotic pathway by ACR was evidenced by the events of loss of mitochondrial membrane potential, bax/bcl-2 dysregulation, cytochrome c release, and activation of caspase-3. Interestingly, PSG-1-F₂ was able to suppress ACR toxicity by improving the redox status of IEC-6 cells and by attenuating mitochondria-mediated apoptosis. Its protective effect was even superior to the clinically used antioxidant N-acetylcysteine (NAC). This study uniquely introduces PSG-1-F₂ as a potential inhibitor of ACR-induced stress and toxicities.
Agid:
6177406