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A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

Author:
Kviatkovski, I., Yarnitzky, T., Shushan, S., Schwartz-Harari, O., Nir-Paz, R., Helman, Y.
Source:
Chemical communications 2018 v.54 no.66 pp. 9218-9221
ISSN:
1364-548X
Subject:
Pseudomonas aeruginosa, Vibrio fischeri, amino acids, biomarkers, biosensors, blood, chemical compounds, chemical reactions, detection limit, genetic engineering, luminescence, lungs, quorum sensing, secondary infection
Abstract:
2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.
Agid:
6177625