PubAg

Main content area

Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin

Author:
Hegemann, Julian D., Schwalen, Christopher J., Mitchell, Douglas A., van der Donk, Wilfred A.
Source:
Chemical communications 2018 v.54 no.65 pp. 9007-9010
ISSN:
1364-548X
Subject:
biosynthesis, chemical compounds, macrocyclization reactions, proteolysis
Abstract:
Substrate binding assays, in vitro proteolytic processing assays, and heterologous lasso peptide production were used to investigate the roles of conserved precursor peptide residues during paeninodin maturation. Specifically, we delineate which residues are important for substrate recognition, proteolysis, and lasso peptide macrocyclization.
Agid:
6177798