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Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin
- Hegemann, Julian D., Schwalen, Christopher J., Mitchell, Douglas A., van der Donk, Wilfred A.
- Chemical communications 2018 v.54 no.65 pp. 9007-9010
- biosynthesis, chemical compounds, macrocyclization reactions, proteolysis
- Substrate binding assays, in vitro proteolytic processing assays, and heterologous lasso peptide production were used to investigate the roles of conserved precursor peptide residues during paeninodin maturation. Specifically, we delineate which residues are important for substrate recognition, proteolysis, and lasso peptide macrocyclization.