Main content area

Complete genome sequence of Lactococcus lactis subsp. lactis SLPE1-3, a novel lactic acid bacterium causing postharvest decay of the mushroom Pleurotus eryngii

Zhao, Yancun, Zhu, Runjie, Wang, Ying, Song, Zhiwei, Shan, Chengjun, Qiu, Jingping, Liu, Fenquan
Journal of plant pathology 2018 v.100 no.3 pp. 467-476
Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, Pleurotus eryngii, biogenesis, cell walls, chromosomes, enzymes, genes, lactic acid bacteria, lipid metabolism, mushrooms, nucleotide sequences, plasmids, postharvest diseases
Lactococcus lactis subsp. lactis is a pathogenic bacterium causing postharvest decay of the cultivated mushroom Pleurotus eryngii, whose pathogenic mechanism is little known. Sequencing of its complete genome is a prerequisite for revealing the molecular mechanism of infection. In this research, the complete genome of SLPE1-3 was obtained using the Single Molecular Real Time (SMRT) sequencing strategy. The genome was analyzed both structurally and functionally. The complete genome of SLPE1-3 consists of a single, circular chromosome (2,522,493 bp; 34.91% GC content) without any plasmid. The results showed the feasibility and superiority of SMRT in bacterial complete-genome research. The genome of SLPE1-3 has the specific features of L. lactis subsp. lactis not just in the phylogenesis and genome structure, but also in functional classification. Compared with L. lactis subsp. lactis IL1403, L. lactis subsp. cremoris MG1363 and L. lactis subsp. lactis KF147, 23 peculiar genes were identified in SLPE1-3 which were involved in lipid metabolism, cell wall biogenesis and some functional enzymes. In addition, 37 potential genes relating to antifungal function were filtered for further mechanism research.