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Soil acidification amendments change the rhizosphere bacterial community of tobacco in a bacterial wilt affected field

Author:
Shen, Guihua, Zhang, Shuting, Liu, Xiaojiao, Jiang, Qipeng, Ding, Wei
Source:
Applied microbiology and biotechnology 2018 v.102 no.22 pp. 9781-9791
ISSN:
0175-7598
Subject:
Aeromicrobium, Pseudoxanthomonas, bacterial communities, bacterial wilt, biochar, continuous cropping, crops, disease control, disease incidence, oyster shells, rhizosphere, soil acidification, soil amendments, soil bacteria, soil pH, tobacco
Abstract:
Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuous cropping and soil acidification. In this study, biochar (CP), lime (LM), oyster shell powder (OS) and no soil amendment additions (Control; CK) were assessed for their abilities to improve the soil acidification, change the composition of rhizosphere soil bacterial communities and thus control tobacco bacterial wilt. The results showed that oyster shell powder significantly increased soil pH by 0.77 and reduced the incidence of tobacco bacterial wilt by 36.67% compared to the control. The Illumina sequencing -based community analysis showed that soil amendment applications affected the composition of rhizosphere bacterial community and increased the richness and diversity. In contrast, the richness and diversity correlated negatively to disease incidence. Using LEfSe analyses, 11 taxa were found to be closely related with disease suppression, in which Saccharibacteria, Aeromicrobium, and Pseudoxanthomonas could be potential indicators of disease suppression. Our results suggested that the suppression of bacterial wilt after the application of soil amendments (especially oyster shell powder) was attributed to the improved soil pH and increased bacterial richness and diversity.
Agid:
6178327