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A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis

Author:
Aono, Akio, Chikamatsu, Kinuyo, Yamada, Hiroyuki, Igarashi, Yuriko, Murase, Yoshiro, Takaki, Akiko, Mitarai, Satoshi
Source:
Journal of microbiological methods 2018 v.154 pp. 52-54
ISSN:
0167-7012
Subject:
Mycobacterium tuberculosis, drugs, enzyme activity, enzymes, liquids, phenotype
Abstract:
We modified Wayne's pyrazinamidase test against Mycobacterium tuberculosis to indirectly measure pyrazinamidase activity via pyrazinoic acid in liquid medium. The modified pyrazinamidase test was easy to perform and its results were in complete agreement with those of the conventional Wayne's method, highlighting its potential application in phenotypic pyrazinamide susceptibility testing.
Agid:
6178927