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A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples
- Liu, Yi H., Chen, Jian, Guo, Yi R., Wang, Chun M., Liang, Xiao, Zhu, Guo N.
- Journal of environmental science and health 2011 v.46 no.4 pp. 313
- agricultural products, chlorpyrifos, detection limit, enzyme-linked immunosorbent assay, inhibitory concentration 50
- A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. The IC₅₀ and IC₁₀ of this ELISA were 3.3 ng/mL and 0.1 ng/mL respectively. The average recoveries in six agricultural products were between 79.5% and 118.0%, with the intra-assay coefficient of variation being less than 8 %. The limit of detection for all tested products was 30 ng/g. To the best of our knowledge, this assay has the best specificity among all the published research on ELISAs for chlorpyrifos.